Composite
Part:BBa_K2760019:Experience
Designed by: iGEM18_TecMonterrey_GDL Group: iGEM18_TecMonterrey_GDL (2018-10-10)
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K2760019
The aplication of this signal peptide is meant to be attached to a recombinant protein to enable their secretion.
This particular construct was created to characterize the efficiency of the signal peptide DsbAss.
The team iGEM18 Tec GDL managed to run western blots and electrophoresis as a way to characterize this construct. (http://2018.igem.org/Team:TecMonterrey_GDL/GeneticE). Since it has a His-Tag, it was detected on the Western Blot assays.
The following image contains our Western blot results from the media protein extraction. http://2018.igem.org/File:T--TecMonterrey_GDL--GenericE--8.png
User Reviews
UNIQ34f98aacd13abab1-partinfo-00000000-QINU UNIQ34f98aacd13abab1-partinfo-00000001-QINU